ID   HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3
AC   CVCL_D4BX
SY   Syn10 Galectin-3 FRET
DR   Wikidata; Q127381683
CC   Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line).
CC   Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission).
CC   Genetic integration: Method=Transduction; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transduction; Gene=FPbase; MZMFB; mRuby3 (Note=Bright red fluorescent protein derived from eqFP611).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:6563; LGALS3.
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11138; SNCA (Note=With p.Ala53Thr).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
CC   Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D4BW ! HEK293T TetOff-SNCA-A53T
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 4
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