Cellosaurus Flp-In-T-REx-293 eGFP-mHCN2 (CVCL_D4B1)
Cell line name | Flp-In-T-REx-293 eGFP-mHCN2 |
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Accession | CVCL_D4B1 |
Resource Identification Initiative | To cite this cell line use: Flp-In-T-REx-293 eGFP-mHCN2 (RRID:CVCL_D4B1) |
Comments | Characteristics: Using pcDNA5/FRT/TO and pOG44, mouse Hcn2 N-terminally tagged with EGFP was integrated in a single site and its expression is regulated by tetracycline (PubMed=38377199). Transfected with: MGI; MGI:1298210; Hcn2. Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P00722; Escherichia coli lacZ. Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_U427 (Flp-In-T-REx-293) |
Sex of cell | Female |
Age at sampling | Fetus |
Category | Transformed cell line |
Publications | PubMed=38377199; DOI=10.1073/pnas.2315132121 |
Entry history | |
Entry creation | 30-Jan-2024 |
Last entry update | 02-May-2024 |
Version number | 2 |