ID   Flp-In-T-REx-293 eGFP-mHCN2
AC   CVCL_D4B1
DR   Wikidata; Q127380859
RX   PubMed=38377199;
CC   Characteristics: Using pcDNA5/FRT/TO and pOG44, mouse Hcn2 N-terminally tagged with eGFP was integrated in a single site and its expression is regulated by tetracycline (PubMed=38377199).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:1298210; Hcn2.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_U427 ! Flp-In-T-REx-293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 30-01-24; Last updated: 10-04-25; Version: 5
//
RX   PubMed=38377199; DOI=10.1073/pnas.2315132121; PMCID=PMC10907242;
RA   Kuschke S., Thon S., Sattler C., Schwabe T., Benndorf K.,
RA   Schmauder R.;
RT   "cAMP binding to closed pacemaker ion channels is cooperative.";
RL   Proc. Natl. Acad. Sci. U.S.A. 121:e2315132121.1-e2315132121.8(2024).
//