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Cellosaurus RN46A (CVCL_D261)

[Text version]
Cell line name RN46A
Synonyms RN-46A
Accession CVCL_D261
Resource Identification Initiative To cite this cell line use: RN46A (RRID:CVCL_D261)
Comments Characteristics: At the restrictive temperature (39 Celsius) the cells are capable of differentiating.
Doubling time: 19 hours (ECACC=12061302).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Derived from site: In situ; Embryonic brain, medullary raphe nucleus; UBERON=UBERON_0002692.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Sprague Dawley; RS=RS_0000681.
Hierarchy Children:
CVCL_U016 (RN46A-B14)
Sex of cell Sex unspecified
Age at sampling 13FD
Category Transformed cell line
Publications

PubMed=7965075; DOI=10.1523/JNEUROSCI.14-11-06744.1994; PMCID=PMC6577239
Linda A. White, Mary J. Eaton, Marcia C. Castro, K. John Klose, Mordecai Yom-Tov Globus, Gerry Shaw, Scott R. Whittemore;
Distinct regulatory pathways control neurofilament expression and neurotransmitter synthesis in immortalized serotonergic neurons.
J. Neurosci. 14:6744-6753(1994)

PubMed=7601307; DOI=10.1006/dbio.1995.1205
Mary J. Eaton, Julie K. Staley, Mordecai Yom-Tov Globus, Scott R. Whittemore;
Developmental regulation of early serotonergic neuronal differentiation: the role of brain-derived neurotrophic factor and membrane depolarization.
Dev. Biol. 170:169-182(1995)

PubMed=8690054; DOI=10.1006/exnr.1996.0121
Mary J. Eaton, Scott R. Whittemore;
Autocrine BDNF secretion enhances the survival and serotonergic differentiation of raphe neuronal precursor cells grafted into the adult rat CNS.
Exp. Neurol. 140:105-114(1996)

PubMed=22863662; DOI=10.1111/j.1582-4934.2012.01607.x; PMCID=PMC4118228
Asir Alwin Prem Anand, S. Gowri Sankar, Kokila V. Vani;
Immortalization of neuronal progenitors using SV40 large T antigen and differentiation towards dopaminergic neurons.
J. Cell. Mol. Med. 16:2592-2610(2012)

Cross-references
Cell line collections (Providers) ECACC; 12061302
Anatomy/cell type resources BTO; BTO_0003779
Encyclopedic resources Wikidata; Q54950749
Experimental variables resources EFO; EFO_0022804
Entry history
Entry creation22-Oct-2012
Last entry update14-Aug-2025
Version number17