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Cellosaurus MDA-MB-231-luc-D3H2LN (CVCL_D257)

[Text version]
Cell line name MDA-MB-231-luc-D3H2LN
Synonyms D3H2LN
Accession CVCL_D257
Resource Identification Initiative To cite this cell line use: MDA-MB-231-luc-D3H2LN (RRID:CVCL_D257)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: Caucasian.
Characteristics: Expression of firefly luciferase is under the control of a SV40 promoter (Revvity/PerkinElmer=119369).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Discontinued: Revvity/PerkinElmer; 119369; true.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1D88 (MDA-MB-231-luc-D3H1)
Sex of cell Female
Age at sampling 51Y
Category Cancer cell line
Web pages Provider; Revvity/PerkinElmer; 119369; https://web.archive.org/web/20150202002416/http://www.perkinelmer.com/catalog/product/id/119369
Publications

PubMed=15987449; DOI=10.1186/bcr1026; PMCID=PMC1175057
Darlene E. Jenkins, Yvette S. Hornig, Yoko A. Oei, Joan M. Dusich, Tony Purchio;
Bioluminescent human breast cancer cell lines that permit rapid and sensitive in vivo detection of mammary tumors and multiple metastases in immune deficient mice.
Breast Cancer Res. 7:R444-R454(2005)

Cross-references
Cell line collections (Providers) Revvity/PerkinElmer; 119369
Cell line databases/resources cancercelllines; CVCL_D257
Encyclopedic resources Wikidata; Q54904599
Entry history
Entry creation22-Oct-2012
Last entry update14-Aug-2025
Version number30