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Cellosaurus A549-AC (CVCL_D1JI)

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Cell line name A549-AC
Accession CVCL_D1JI
Resource Identification Initiative To cite this cell line use: A549-AC (RRID:CVCL_D1JI)
Comments Group: SARS-CoV-2 research cell line.
Population: Caucasian.
Characteristics: Expresses ACE2 under the control of the EF1a promoter (from parent cell line).
Characteristics: Transduced with a EF1a-Cas9-2A-eGFP vector (PubMed=36032971).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:13557; ACE2.
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Omics: Phenotyping; CRISPR screening.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A7UV (A549-hACE2 [BEI resources])
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Publications

PubMed=36032971; DOI=10.21203/rs.3.rs-1910932/v1; PMCID=PMC9413708
Jia-Kai Hou, Yan-Jun Wei, Jing Zou, Roshni Jaffery, Shao-Heng Liang, Cai-Shang Zheng, Ken Chen, Pei-Yong Shi, Yi-Wen Chen, Xu-Ping Xie, Wei-Yi Peng;
Integrated multi-omics analyses identify key anti-viral host factors and pathways controlling SARS-CoV-2 infection.
Res. Sq. 2022:1910932-1910932(2022)

Cross-references
Encyclopedic resources Wikidata; Q127378820
Entry history
Entry creation30-Jan-2024
Last entry update14-Aug-2025
Version number5