Cellosaurus cell line NS-5 (CVCL

Cellosaurus NS-5 (CVCL_D1HI)

Cross-references
Cell line name	NS-5
Synonyms	NS5
Accession	CVCL_D1HI
Resource Identification Initiative	To cite this cell line use: NS-5 (RRID:CVCL_D1HI)
Comments	Characteristics: Can differentiate into both astrocytes and neurons (PubMed=16086633). Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line). Knockout cell: Method=Homologous recombination; RGD; 6487636; Sox1. Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG). Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Neural stem cell; CL=CL_0000047.
Species of origin	Mus musculus (Mouse) (NCBI Taxonomy: 10090) Breed/subspecies: 129P2/Ola.
Hierarchy	Parent: CVCL_Y482 (46C)
Sex of cell	Male
Age at sampling	Blastocyst stage
Category	Somatic stem cell
Publications	PubMed=16086633; DOI=10.1371/journal.pbio.0030283; PMCID=PMC1184591 Luciano Conti, Steven M. Pollard, Thorsten Gorba, Erika Reitano, Mauro Toselli, Gerardo Biella, Yi-Rui Sun, Sveva Sanzone, Qi-Long Ying, Elena Cattaneo, Austin Gerard Smith; Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol. 3:e283.1594-e283.1606(2005) PubMed=20626555; DOI=10.1111/j.1471-4159.2010.06886.x Steven Daniel Hicks, Frank A. Middleton, Michael W. Miller; Ethanol-induced methylation of cell cycle genes in neural stem cells. J. Neurochem. 114:1767-1780(2010) PubMed=30748015; DOI=10.1111/acer.13976 Michael W. Miller; p53-mediated activities in NS-5 neural stem cells: effects of ethanol. Alcohol. Clin. Exp. Res. 43:655-667(2019)
Anatomy/cell type resources	BTO; BTO_0006551
Encyclopedic resources	Wikidata; Q127382972
Entry history
Entry creation	30-Jan-2024
Last entry update	14-Aug-2025
Version number	5