ID   NS-5
SY   NS5
DR   BTO; BTO:0006551
RX   PubMed=16086633;
RX   PubMed=20626555;
RX   PubMed=30748015;
CC   Characteristics: Can differentiate into both astrocytes and neurons (PubMed=16086633).
CC   Characteristics: Expresses eGFP under the control of the Sox-1 promoter (from parent cell line).
CC   Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Neural stem cell; CL=CL_0000047.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_Y482 ! 46C
SX   Male
AG   Blastocyst stage
CA   Somatic stem cell
DT   Created: 30-01-24; Last updated: 30-01-24; Version: 1
RX   PubMed=16086633; DOI=10.1371/journal.pbio.0030283;
RA   Conti L., Pollard S.M., Gorba T., Reitano E., Toselli M., Biella G.,
RA   Sun Y.-R., Sanzone S., Ying Q.-L., Cattaneo E., Smith A.G.;
RT   "Niche-independent symmetrical self-renewal of a mammalian tissue stem
RT   cell.";
RL   PLoS Biol. 3:e283.1594-e283.1606(2005).
RX   PubMed=20626555; DOI=10.1111/j.1471-4159.2010.06886.x;
RA   Hicks S.D., Middleton F.A., Miller M.W.;
RT   "Ethanol-induced methylation of cell cycle genes in neural stem
RT   cells.";
RL   J. Neurochem. 114:1767-1780(2010).
RX   PubMed=30748015; DOI=10.1111/acer.13976;
RA   Miller M.W.;
RT   "p53-mediated activities in NS-5 neural stem cells: effects of
RT   ethanol.";
RL   Alcohol. Clin. Exp. Res. 43:655-667(2019).