ID   NS-5
AC   CVCL_D1HI
SY   NS5
DR   BTO; BTO_0006551
DR   Wikidata; Q127382972
RX   PubMed=16086633;
RX   PubMed=20626555;
RX   PubMed=30748015;
CC   Characteristics: Can differentiate into both astrocytes and neurons (PubMed=16086633).
CC   Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line).
CC   Knockout cell: Method=Homologous recombination; RGD; 6487636; Sox1.
CC   Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
CC   Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Neural stem cell; CL=CL_0000047.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_Y482 ! 46C
SX   Male
AG   Blastocyst stage
CA   Somatic stem cell
DT   Created: 30-01-24; Last updated: 14-08-25; Version: 5
//
RX   PubMed=16086633; DOI=10.1371/journal.pbio.0030283; PMCID=PMC1184591;
RA   Conti, Luciano
RA   Pollard, Steven M.
RA   Gorba, Thorsten
RA   Reitano, Erika
RA   Toselli, Mauro
RA   Biella, Gerardo
RA   Sun, Yi-Rui
RA   Sanzone, Sveva
RA   Ying, Qi-Long
RA   Cattaneo, Elena
RA   Smith, Austin Gerard
RT   "Niche-independent symmetrical self-renewal of a mammalian tissue stem
RT   cell.";
RL   PLoS Biol. 3:e283.1594-e283.1606(2005).
//
RX   PubMed=30748015; DOI=10.1111/acer.13976;
RA   Miller, Michael W.
RT   "p53-mediated activities in NS-5 neural stem cells: effects of
RT   ethanol.";
RL   Alcohol. Clin. Exp. Res. 43:655-667(2019).
//
RX   PubMed=20626555; DOI=10.1111/j.1471-4159.2010.06886.x;
RA   Hicks, Steven Daniel
RA   Middleton, Frank A.
RA   Miller, Michael W.
RT   "Ethanol-induced methylation of cell cycle genes in neural stem
RT   cells.";
RL   J. Neurochem. 114:1767-1780(2010).
//