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Cellosaurus BA/F3/Cas9-hyg (CVCL_C9FI)

[Text version]
Cell line name BA/F3/Cas9-hyg
Accession CVCL_C9FI
Resource Identification Initiative To cite this cell line use: BA/F3/Cas9-hyg (RRID:CVCL_C9FI)
Comments Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL567).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Caution: The parent cell line was originally thought to originate from a BALB/c mouse but is in fact from a C3H mouse.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Cell type: Pro-B cell; CL=CL_0000826.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H.
Hierarchy Parent: CVCL_0161 (Ba/F3)
Category Factor-dependent cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL567
Encyclopedic resources Wikidata; Q123030760
Entry history
Entry creation05-Oct-2023
Last entry update30-Jan-2024
Version number2