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Cellosaurus Neuro-2a/HF-Cas9-Rosa26 (CVCL_C9FB)

[Text version]
Cell line name Neuro-2a/HF-Cas9-Rosa26
Synonyms Neuro 2a/HF-Cas9-ROSA26
Accession CVCL_C9FB
Resource Identification Initiative To cite this cell line use: Neuro-2a/HF-Cas9-Rosa26 (RRID:CVCL_C9FB)
Comments Characteristics: Stably expresses a CMV-3xFLAG-HFCas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the Rosa26 safe locus (GeneCopoeia=SL571).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asn497Ala, p.Arg661Ala, p.Gln695Ala and p.Gln926Ala = High-fidelity Cas9).
Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (copGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
Cell type: Neural crest derived neuroblast; CL=CL_0002676.
Disease Mouse neuroblastoma (NCIt: C21993)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: A/J.
Hierarchy Parent: CVCL_0470 (Neuro-2a)
Sex of cell Male
Category Cancer cell line
Cell line collections (Providers) GeneCopoeia; SL571
Encyclopedic resources Wikidata; Q123033167
Entry history
Entry creation05-Oct-2023
Last entry update30-Jan-2024
Version number2