Cellosaurus cell line HT1080/Cas9 AAVS1 (CVCL

Cross-references
Cell line name	HT1080/Cas9 AAVS1
Synonyms	HT1080-Cas9 AAVS1
Accession	CVCL_C9F8
Resource Identification Initiative	To cite this cell line use: HT1080/Cas9 AAVS1 (RRID:CVCL_C9F8)
Comments	Population: Caucasian. Characteristics: Stably expresses a CBh-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL512). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (copGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: In situ; Bone, pelvis, acetabulum; UBERON=UBERON_0001269.
Sequence variations	Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease	Fibrosarcoma (NCIt: C3043) Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0317 (HT-1080)
Sex of cell	Male
Age at sampling	35Y
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL512
Encyclopedic resources	Wikidata; Q123032637
Entry history
Entry creation	05-Oct-2023
Last entry update	30-Jan-2024
Version number	2