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Cellosaurus KP-Cas9-Clone 7 (CVCL_C9B0)

[Text version]
Cell line name KP-Cas9-Clone 7
Accession CVCL_C9B0
Resource Identification Initiative To cite this cell line use: KP-Cas9-Clone 7 (RRID:CVCL_C9B0)
Comments Knockout cell: Method=KO mouse; MGI; MGI:98834; Trp53.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Genetic integration: Method=Transgenic mouse; Gene=MGI; MGI:96680; Kras (Note=With p.Gly12Asp).
Omics: Phenotyping; CRISPR screening.
Disease Adenocarcinoma of the mouse pulmonary system (NCIt: C21771)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6 KrasLSL-G12D/+, Trp53flox/flox (KP).
Hierarchy Parent: CVCL_C9AY (KP-Clone 7)
Category Cancer cell line
Publications

PubMed=31744829; DOI=10.1158/2159-8290.CD-19-0780; PMCID=PMC7007372
Li F., Huang Q.-Y., Luster T.A., Hu H., Zhang H., Ng W.-L., Khodadadi-Jamayran A., Wang W., Chen T., Deng J.-H., Ranieri M., Fang Z.-Y., Pyon V., Dowling C.M., Bagdatlioglu E., Almonte C., Labbe K., Silver H., Rabin A.R., Jani K., Tsirigos A., Papagiannakopoulos T.Y., Hammerman P.S., Velcheti V., Freeman G.J., Qi J., Miller G., Wong K.-K.
In vivo epigenetic CRISPR screen identifies Asf1a as an immunotherapeutic target in Kras-mutant lung adenocarcinoma.
Cancer Discov. 10:270-287(2020)

Cross-references
CRISP screens repositories BioGRID_ORCS_Cell_line; 1122
Encyclopedic resources Wikidata; Q123032845
Entry history
Entry creation05-Oct-2023
Last entry update10-Apr-2025
Version number5