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Cellosaurus HEKzeroT (CVCL_C8CF)

[Text version]
Cell line name HEKzeroT
Synonyms clone #8
Accession CVCL_C8CF
Resource Identification Initiative To cite this cell line use: HEKzeroT (RRID:CVCL_C8CF)
Comments Group: Serum/protein free medium cell line.
Characteristics: Using CRISPR/Cas9 the SV40 large and small T-antigen coding sequences were deleted thus rendering the cell line CMC compliant (PubMed=37232541).
Characteristics: Grows in suspension growth conditions in animal component-free media.
Doubling time: ~20 hours (PubMed=37232541).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C8CE (HEKExpress)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages https://excellgene.com/services/hek-zero-t/

PubMed=37232541; DOI=10.1002/bit.28414
Croissant C., Armitano J., Lazuech B., Svec D., Pugin C., Guesdon A., Bryan L., Castro A., Neuhaus L., Fonti G., Martinis J., Wurm M.J., Wurm F.M., Pino P.
A new T-antigen negative HEK293 cell line with improved AAV productivity.
Biotechnol. Bioeng. 120:1953-1960(2023)

Encyclopedic resources Wikidata; Q123031597
Entry history
Entry creation29-Jun-2023
Last entry update30-Jan-2024
Version number3