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Cellosaurus WAe009-A-92 (CVCL_C4RV)

[Text version]
Cell line name WAe009-A-92
Synonyms Tet-on-ETV2 H9; NCCDFWe010-A-92
Accession CVCL_C4RV
Resource Identification Initiative To cite this cell line use: WAe009-A-92 (RRID:CVCL_C4RV)
Comments From: National Center for Cardiovascular Diseases and Fuwai Hospital; Beijing; China.
Characteristics: Expresses ETV2 induced by doxycyclin. Transduced with a Tre-hETV2-mPGK-PuroR-rtTA-T2A-EF1a construct that contains the reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=36463633).
Transfected with: HGNC; 3491; ETV2.
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=36463633; DOI=10.1016/j.scr.2022.102985
Zhou M., Ni J., Huang P.-F., Liu X.-J.
Generation of a doxycycline-inducible ETV2 expression cell line using PiggyBac transposase system.
Stem Cell Res. 66:102985-102985(2023)

Cross-references
Cell line databases/resources hPSCreg; WAe009-A-92
Biological sample resources BioSamples; SAMEA110358604
Encyclopedic resources Wikidata; Q116050776
Entry history
Entry creation15-Dec-2022
Last entry update29-Jun-2023
Version number3