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Cellosaurus IZD-MB-0503 (CVCL_C411)

[Text version]
Cell line name IZD-MB-0503
Synonyms IZD-Mb-0503; IZD-Mb 0503; IZD-MB0503; IZD-Mb0503; MB-0503; Mb-0503; Mb 0503; MB0503
Accession CVCL_C411
Resource Identification Initiative To cite this cell line use: IZD-MB-0503 (RRID:CVCL_C411)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Problematic cell line: Misidentified. Originally thought to be of Mamestra brassicae origin but found to be from Trichoplusia ni (PubMed=8799737). Could be identical to TN-CL1 (Cellosaurus=CVCL_Z257). Originally thought to originate from larval hemocytes.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00603.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8003.
Doubling time: 12-14 hours (DOI=10.1111/j.1439-0418.1976.tb03417.x); 30 +- 2 hours (Note=In static culture), 24 +- 1 hours (Note=In suspension culture) (PubMed=10585188).
Misspelling: IZB Mb 0507; PubMed=1369220.
Species of origin Trichoplusia ni (Cabbage looper) (NCBI Taxonomy: 7111)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Spontaneously immortalized cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

DOI=10.1111/j.1439-0418.1976.tb03417.x
Herbert Georg Miltenburger, P. David, Ulrike Mahr, W. Zipp;
Establishment of lepidopteran cell lines and in vitro replication of insect-pathogenic viruses. I. Mamestra brassicae cell lines and NPV replication.
J. Appl. Entomol. 82:306-323(1977)

CLPUB00496
Walter Frederic Hink;
The 1979 compilation of invertebrate cell lines and culture media.
(In book chapter) Invertebrate systems in vitro; Kurstak, Edouard & Maramorosch, Karl & Dubendorfer, Andreas (eds.); pp.553-578; Elsevier; Oxford; United Kingdom (1980)

PubMed=1367489; DOI=10.1021/bp00007a002
Walter Frederic Hink, Darrell R. Thomsen, Don J. Davidson, Annette L. Meyer, Francis J. Castellino;
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
Biotechnol. Prog. 7:9-14(1991)

PubMed=1369220; DOI=10.1021/bp00017a003
Thomas J. Wickham, Thomas R. Davis, Robert R. Granados, Michael Louis Shuler, H. Alan Wood;
Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
Biotechnol. Prog. 8:391-396(1992)

PubMed=8314732; DOI=10.1007/BF02633986
Thomas R. Davis, Thomas J. Wickham, Kevin A. McKenna, Robert R. Granados, Michael Louis Shuler, H. Alan Wood;
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
Arthur H. McIntosh, James J. Grasela, Robert L. Matteri;
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)

PubMed=10585188; DOI=10.1021/bp990119f
Michelle B. Keith, Patrick J. Farrell, Kostas Iatrou, Leo Augustus Behie;
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

PubMed=12383871; DOI=10.1016/S0196-9781(02)00140-7
Davide Malagoli, Mauro Mandrioli, Enzo Ottaviani;
Cloning and characterisation of a procorticotrophin-releasing hormone in the IZD-MB-0503 immunocyte line from the insect Mamestra brassicae.
Peptides 23:1829-1836(2002)

PubMed=12459194; DOI=10.1016/S0965-1748(02)00189-3
Mauro Mandrioli, Ernst A. Wimmer;
Stable transformation of a Mamestra brassicae (lepidoptera) cell line with the lepidopteran-derived transposon piggyBac.
Insect Biochem. Mol. Biol. 33:1-5(2003)

PubMed=12753953; DOI=10.1016/S0248-4900(02)01219-4
Mauro Mandrioli, Silvia Bugli, Sabrina Saltini, Susanna Genedani, Enzo Ottaviani;
Molecular characterization of a defensin in the IZD-MB-0503 cell line derived from immunocytes of the insect Mamestra brassicae (Lepidoptera).
Biol. Cell 95:53-57(2003)

PubMed=14620958; DOI=10.1023/A:1026016021415
Mauro Mandrioli, Nicola Volpi;
The genome of the lepidopteran Mamestra brassicae has a vertebrate-like content of methyl-cytosine.
Genetica 119:187-191(2003)

Cross-references
Cell line collections (Providers) ECACC; 93090714 - Discontinued
Cell line databases/resources CLO; CLO_0006980
ICLDB; izd-mb-0503
Encyclopedic resources Wikidata; Q54898396
Entry history
Entry creation22-Oct-2012
Last entry update14-Aug-2025
Version number16