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Cellosaurus IZD-MB-0503 (CVCL_C411)

[Text version]
Cell line name IZD-MB-0503
Synonyms IZD-Mb-0503; IZD-Mb 0503; IZD-MB0503; IZD-Mb0503; MB-0503; Mb-0503; Mb 0503; MB0503
Accession CVCL_C411
Resource Identification Initiative To cite this cell line use: IZD-MB-0503 (RRID:CVCL_C411)
Comments Group: Insect cell line.
Group: Recombinant protein production insect cell line.
Problematic cell line: Misidentified. Originally thought to be of Mamestra brassicae origin but found to be from Trichoplusia ni (PubMed=8799737). Could be identical to TN-CL1 (Cellosaurus=CVCL_Z257). Originally thought to originate from larval hemocytes.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00603.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8003.
Doubling time: 12-14 hours (DOI=10.1111/j.1439-0418.1976.tb03417.x); 30 +- 2 hours (Note=In static culture), 24 +- 1 hours (Note=In suspension culture) (PubMed=10585188).
Misspelling: IZB Mb 0507; PubMed=1369220.
Species of origin Trichoplusia ni (Cabbage looper) (NCBI Taxonomy: 7111)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Spontaneously immortalized cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx

Miltenburger H.G., David P., Mahr U., Zipp W.
Establishment of lepidopteran cell lines and in vitro replication of insect-pathogenic viruses. I. Mamestra brassicae cell lines and NPV replication.
J. Appl. Entomol. 82:306-323(1977)

Hink W.F.
The 1979 compilation of invertebrate cell lines and culture media.
(In) Invertebrate systems in vitro; Kurstak E., Maramorosch K., Dubendorfer A. (eds.); pp.553-578; Elsevier; Oxford (1980)

PubMed=1367489; DOI=10.1021/bp00007a002
Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines.
Biotechnol. Prog. 7:9-14(1991)

PubMed=1369220; DOI=10.1021/bp00017a003
Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.
Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system.
Biotechnol. Prog. 8:391-396(1992)

PubMed=8314732; DOI=10.1007/BF02633986
Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L., Wood H.A.
Comparative recombinant protein production of eight insect cell lines.
In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993)

PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
McIntosh A.H., Grasela J.J., Matteri R.L.
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)

PubMed=10585188; DOI=10.1021/bp990119f
Keith M.B., Farrell P.J., Iatrou K., Behie L.A.
Screening of transformed insect cell lines for recombinant protein production.
Biotechnol. Prog. 15:1046-1052(1999)

PubMed=12383871; DOI=10.1016/S0196-9781(02)00140-7
Malagoli D., Mandrioli M., Ottaviani E.
Cloning and characterisation of a procorticotrophin-releasing hormone in the IZD-MB-0503 immunocyte line from the insect Mamestra brassicae.
Peptides 23:1829-1836(2002)

PubMed=12459194; DOI=10.1016/S0965-1748(02)00189-3
Mandrioli M., Wimmer E.A.
Stable transformation of a Mamestra brassicae (lepidoptera) cell line with the lepidopteran-derived transposon piggyBac.
Insect Biochem. Mol. Biol. 33:1-5(2003)

PubMed=12753953; DOI=10.1016/S0248-4900(02)01219-4
Mandrioli M., Bugli S., Saltini S., Genedani S., Ottaviani E.
Molecular characterization of a defensin in the IZD-MB-0503 cell line derived from immunocytes of the insect Mamestra brassicae (Lepidoptera).
Biol. Cell 95:53-57(2003)

PubMed=14620958; DOI=10.1023/A:1026016021415
Mandrioli M., Volpi N.
The genome of the lepidopteran Mamestra brassicae has a vertebrate-like content of methyl-cytosine.
Genetica 119:187-191(2003)

Cell line collections (Providers) ECACC; 93090714 - Discontinued
Cell line databases/resources CLO; CLO_0006980
ICLDB; izd-mb-0503
Encyclopedic resources Wikidata; Q54898396
Entry history
Entry creation22-Oct-2012
Last entry update02-May-2024
Version number14