ID   IZD-MB-0503
AC   CVCL_C411
SY   IZD-Mb-0503; IZD-Mb 0503; IZD-MB0503; IZD-Mb0503; MB-0503; Mb-0503; Mb 0503; MB0503
DR   CLO; CLO_0006980
DR   ECACC; 93090714
DR   ICLDB; izd-mb-0503
DR   Wikidata; Q54898396
RX   CelloPub=CLPUB00496;
RX   DOI=10.1111/j.1439-0418.1976.tb03417.x;
RX   PubMed=787805;
RX   PubMed=1367489;
RX   PubMed=1369220;
RX   PubMed=8314732;
RX   PubMed=8799737;
RX   PubMed=10585188;
RX   PubMed=12383871;
RX   PubMed=12459194;
RX   PubMed=12753953;
RX   PubMed=14620958;
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Group: Insect cell line.
CC   Group: Recombinant protein production insect cell line.
CC   Problematic cell line: Misidentified. Originally thought to be of Mamestra brassicae origin but found to be from Trichoplusia ni (PubMed=8799737). Could be identical to TN-CL1 (Cellosaurus=CVCL_Z257). Originally thought to originate from larval hemocytes.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00603.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8003.
CC   Doubling time: 12-14 hours (DOI=10.1111/j.1439-0418.1976.tb03417.x); 24-26 hours (PubMed=787805); 30 +- 2 hours (Note=In static culture), 24 +- 1 hours (Note=In suspension culture) (PubMed=10585188).
CC   Misspelling: IZB Mb 0507; PubMed=1369220.
CC   Discontinued: ECACC; 93090714; true.
OX   NCBI_TaxID=7111; ! Trichoplusia ni (Cabbage looper)
SX   Sex unspecified
AG   Age unspecified
CA   Spontaneously immortalized cell line
DT   Created: 22-10-12; Last updated: 27-11-25; Version: 17
//
RX   DOI=10.1111/j.1439-0418.1976.tb03417.x;
RA   Miltenburger, Herbert Georg
RA   David, P.
RA   Mahr, Ulrike
RA   Zipp, W.
RT   "Establishment of lepidopteran cell lines and in vitro replication of
RT   insect-pathogenic viruses. I. Mamestra brassicae cell lines and NPV
RT   replication.";
RL   J. Appl. Entomol. 82:306-323(1977).
//
RX   PubMed=1369220; DOI=10.1021/bp00017a003;
RA   Wickham, Thomas J.
RA   Davis, Thomas R.
RA   Granados, Robert R.
RA   Shuler, Michael Louis
RA   Wood, H. Alan
RT   "Screening of insect cell lines for the production of recombinant
RT   proteins and infectious virus in the baculovirus expression system.";
RL   Biotechnol. Prog. 8:391-396(1992).
//
RX   PubMed=1367489; DOI=10.1021/bp00007a002;
RA   Hink, Walter Frederic
RA   Thomsen, Darrell R.
RA   Davidson, Don J.
RA   Meyer, Annette L.
RA   Castellino, Francis J.
RT   "Expression of three recombinant proteins using baculovirus vectors in
RT   23 insect cell lines.";
RL   Biotechnol. Prog. 7:9-14(1991).
//
RX   PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x;
RA   McIntosh, Arthur H.
RA   Grasela, James J.
RA   Matteri, Robert L.
RT   "Identification of insect cell lines by DNA amplification
RT   fingerprinting (DAF).";
RL   Insect Mol. Biol. 5:187-195(1996).
//
RX   PubMed=787805; DOI=10.1007/BF00624227;
RA   Miltenburger, Herbert Georg
RA   David, P.
RT   "Nuclear polyhedrosis virus replication in permanent cell lines of the
RT   cabbage moth (Mamestra brassicae L.).";
RL   Naturwissenschaften 63:197-198(1976).
//
RX   CelloPub=CLPUB00496;
RA   Hink, Walter Frederic
RT   "The 1979 compilation of invertebrate cell lines and culture media.";
RL   (In book chapter) Invertebrate systems in vitro; Kurstak, Edouard & Maramorosch, Karl & Dubendorfer, Andreas (eds.); pp.553-578; Elsevier; Oxford; United Kingdom (1980).
//
RX   PubMed=10585188; DOI=10.1021/bp990119f;
RA   Keith, Michelle B.
RA   Farrell, Patrick J.
RA   Iatrou, Kostas
RA   Behie, Leo Augustus
RT   "Screening of transformed insect cell lines for recombinant protein
RT   production.";
RL   Biotechnol. Prog. 15:1046-1052(1999).
//
RX   PubMed=14620958; DOI=10.1023/A:1026016021415;
RA   Mandrioli, Mauro
RA   Volpi, Nicola
RT   "The genome of the lepidopteran Mamestra brassicae has a
RT   vertebrate-like content of methyl-cytosine.";
RL   Genetica 119:187-191(2003).
//
RX   PubMed=12753953; DOI=10.1016/S0248-4900(02)01219-4;
RA   Mandrioli, Mauro
RA   Bugli, Silvia
RA   Saltini, Sabrina
RA   Genedani, Susanna
RA   Ottaviani, Enzo
RT   "Molecular characterization of a defensin in the IZD-MB-0503 cell line
RT   derived from immunocytes of the insect Mamestra brassicae
RT   (Lepidoptera).";
RL   Biol. Cell 95:53-57(2003).
//
RX   PubMed=8314732; DOI=10.1007/BF02633986;
RA   Davis, Thomas R.
RA   Wickham, Thomas J.
RA   McKenna, Kevin A.
RA   Granados, Robert R.
RA   Shuler, Michael Louis
RA   Wood, H. Alan
RT   "Comparative recombinant protein production of eight insect cell
RT   lines.";
RL   In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993).
//
RX   PubMed=12459194; DOI=10.1016/S0965-1748(02)00189-3;
RA   Mandrioli, Mauro
RA   Wimmer, Ernst A.
RT   "Stable transformation of a Mamestra brassicae (lepidoptera) cell line
RT   with the lepidopteran-derived transposon piggyBac.";
RL   Insect Biochem. Mol. Biol. 33:1-5(2003).
//
RX   PubMed=12383871; DOI=10.1016/S0196-9781(02)00140-7;
RA   Malagoli, Davide
RA   Mandrioli, Mauro
RA   Ottaviani, Enzo
RT   "Cloning and characterisation of a procorticotrophin-releasing hormone
RT   in the IZD-MB-0503 immunocyte line from the insect Mamestra
RT   brassicae.";
RL   Peptides 23:1829-1836(2002).
//