ID   IZD-MB-0503
AC   CVCL_C411
SY   IZD-Mb-0503; IZD-Mb 0503; IZD-MB0503; IZD-Mb0503; MB-0503; Mb-0503; Mb 0503; MB0503
DR   CLO; CLO_0006980
DR   ECACC; 93090714
DR   ICLDB; izd-mb-0503
DR   Wikidata; Q54898396
RX   CelloPub=CLPUB00496;
RX   DOI=10.1111/j.1439-0418.1976.tb03417.x;
RX   PubMed=1369220;
RX   PubMed=1367489;
RX   PubMed=8314732;
RX   PubMed=8799737;
RX   PubMed=10585188;
RX   PubMed=12383871;
RX   PubMed=12459194;
RX   PubMed=12753953;
RX   PubMed=14620958;
CC   Group: Insect cell line.
CC   Group: Recombinant protein production insect cell line.
CC   Problematic cell line: Misidentified. Originally thought to be of Mamestra brassicae origin but found to be from Trichoplusia ni (PubMed=8799737). Could be identical to TN-CL1 (Cellosaurus=CVCL_Z257). Originally thought to originate from larval hemocytes.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00603.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8003.
CC   Doubling time: 12-14 hours (DOI=10.1111/j.1439-0418.1976.tb03417.x); 30 +- 2 hours (Note=In static culture), 24 +- 1 hours (Note=In suspension culture) (PubMed=10585188).
CC   Misspelling: IZB Mb 0507; PubMed=1369220.
CC   Discontinued: ECACC; 93090714; probable.
OX   NCBI_TaxID=7111; ! Trichoplusia ni (Cabbage looper)
SX   Sex unspecified
AG   Age unspecified
CA   Spontaneously immortalized cell line
DT   Created: 22-10-12; Last updated: 02-05-24; Version: 14
RX   CelloPub=CLPUB00496;
RA   Hink W.F.;
RT   "The 1979 compilation of invertebrate cell lines and culture media.";
RL   (In) Invertebrate systems in vitro; Kurstak E., Maramorosch K., Dubendorfer A. (eds.); pp.553-578; Elsevier; Oxford (1980).
RX   DOI=10.1111/j.1439-0418.1976.tb03417.x;
RA   Miltenburger H.G., David P., Mahr U., Zipp W.;
RT   "Establishment of lepidopteran cell lines and in vitro replication of
RT   insect-pathogenic viruses. I. Mamestra brassicae cell lines and NPV
RT   replication.";
RL   J. Appl. Entomol. 82:306-323(1977).
RX   PubMed=1369220; DOI=10.1021/bp00017a003;
RA   Wickham T.J., Davis T., Granados R.R., Shuler M.L., Wood H.A.;
RT   "Screening of insect cell lines for the production of recombinant
RT   proteins and infectious virus in the baculovirus expression system.";
RL   Biotechnol. Prog. 8:391-396(1992).
RX   PubMed=1367489; DOI=10.1021/bp00007a002;
RA   Hink W.F., Thomsen D.R., Davidson D.J., Meyer A.L., Castellino F.J.;
RT   "Expression of three recombinant proteins using baculovirus vectors in
RT   23 insect cell lines.";
RL   Biotechnol. Prog. 7:9-14(1991).
RX   PubMed=8314732; DOI=10.1007/BF02633986;
RA   Davis T.R., Wickham T.J., McKenna K.A., Granados R.R., Shuler M.L.,
RA   Wood H.A.;
RT   "Comparative recombinant protein production of eight insect cell
RT   lines.";
RL   In Vitro Cell. Dev. Biol. Anim. 29:388-390(1993).
RX   PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x;
RA   McIntosh A.H., Grasela J.J., Matteri R.L.;
RT   "Identification of insect cell lines by DNA amplification
RT   fingerprinting (DAF).";
RL   Insect Mol. Biol. 5:187-195(1996).
RX   PubMed=10585188; DOI=10.1021/bp990119f;
RA   Keith M.B., Farrell P.J., Iatrou K., Behie L.A.;
RT   "Screening of transformed insect cell lines for recombinant protein
RT   production.";
RL   Biotechnol. Prog. 15:1046-1052(1999).
RX   PubMed=12383871; DOI=10.1016/S0196-9781(02)00140-7;
RA   Malagoli D., Mandrioli M., Ottaviani E.;
RT   "Cloning and characterisation of a procorticotrophin-releasing hormone
RT   in the IZD-MB-0503 immunocyte line from the insect Mamestra
RT   brassicae.";
RL   Peptides 23:1829-1836(2002).
RX   PubMed=12459194; DOI=10.1016/S0965-1748(02)00189-3;
RA   Mandrioli M., Wimmer E.A.;
RT   "Stable transformation of a Mamestra brassicae (lepidoptera) cell line
RT   with the lepidopteran-derived transposon piggyBac.";
RL   Insect Biochem. Mol. Biol. 33:1-5(2003).
RX   PubMed=12753953; DOI=10.1016/S0248-4900(02)01219-4;
RA   Mandrioli M., Bugli S., Saltini S., Genedani S., Ottaviani E.;
RT   "Molecular characterization of a defensin in the IZD-MB-0503 cell line
RT   derived from immunocytes of the insect Mamestra brassicae
RT   (Lepidoptera).";
RL   Biol. Cell 95:53-57(2003).
RX   PubMed=14620958; DOI=10.1023/A:1026016021415;
RA   Mandrioli M., Volpi N.;
RT   "The genome of the lepidopteran Mamestra brassicae has a
RT   vertebrate-like content of methyl-cytosine.";
RL   Genetica 119:187-191(2003).