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Cellosaurus 409B2 HCN4-EGFP/SHOX2-mCherry (CVCL_C3LU)

[Text version]
Cell line name 409B2 HCN4-EGFP/SHOX2-mCherry
Accession CVCL_C3LU
Resource Identification Initiative To cite this cell line use: 409B2 HCN4-EGFP/SHOX2-mCherry (RRID:CVCL_C3LU)
Comments Population: Caucasian.
Characteristics: Dual reporter cell line. Using homologous recombination EGFP was inserted at the N-terminus of HCN4 and using CRISPR/Cas9 mCherry was inserted at the N-terminus of SHOX2 (PubMed=36092505).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_K092 (409B2)
Sex of cell Female
Age at sampling 36Y
Category Induced pluripotent stem cell
Publications

PubMed=36092505; DOI=10.1016/j.reth.2022.07.012; PMCID=PMC9420958
Takayuki Wakimizu, Kumi Morikawa, Kenta Fukumura, Tetsuo Yuki, Takashi Adachi, Yasutaka Kurata, Junichiro Miake, Ichiro Hisatome, Motokazu Tsuneto, Yasuaki Shirayoshi;
SHOX2 refines the identification of human sinoatrial nodal cell population in the in vitro cardiac differentiation.
Regen. Ther. 21:239-249(2022)

Cross-references
Encyclopedic resources Wikidata; Q116048197
Entry history
Entry creation15-Dec-2022
Last entry update10-Sep-2024
Version number4