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Cellosaurus A375 Safe Harbor Landing Pad (CVCL_C3EX)

[Text version]
Cell line name A375 Safe Harbor Landing Pad
Accession CVCL_C3EX
Resource Identification Initiative To cite this cell line use: A375 Safe Harbor Landing Pad (RRID:CVCL_C3EX)
Comments Population: Caucasian.
Characteristics: Using ZFN a fluorescent mKATE2 tag flanked by Cre-lox sites (LoxN-EF1a-Lox2272-mKATE2-LoxP) was introduced in the AAVS1 safe harbor locus. The mKATE2 tag can be exchanged for a payload of the user's choice using Cre recombinase and a targeting vector with appropriate lox sites (Sigma-Aldrich).
Doubling time: ~22 hours (Sigma-Aldrich).
Transfected with: mKate2, a bright red fluorescent protein derived from eqFP578.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T); ClinVar=VCV000376309; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T); ClinVar=VCV000376308; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-146C>T (c.250C>T) (C250T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0132 (A-375)
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
Web pages https://www.sigmaaldrich.com/catalog/product/sigma/cll1219
Cross-references
Cell line databases/resources cancercelllines; CVCL_C3EX
Encyclopedic resources Wikidata; Q114310508
Entry history
Entry creation22-Sep-2022
Last entry update05-Oct-2023
Version number5