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Cellosaurus THP-1 Safe Harbor Landing Pad (CVCL_C3EW)

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Cell line name THP-1 Safe Harbor Landing Pad
Accession CVCL_C3EW
Resource Identification Initiative To cite this cell line use: THP-1 Safe Harbor Landing Pad (RRID:CVCL_C3EW)
Comments Population: Japanese.
Characteristics: Using ZFN a fluorescent mKATE2 tag flanked by Cre-lox sites (LoxN-EF1a-Lox2272-mKATE2-LoxP) was introduced in the AAVS1 safe harbor locus. The mKATE2 tag can be exchanged for a payload of the user's choice using Cre recombinase and a targeting vector with appropriate lox sites (Sigma-Aldrich=CLL1223).
Doubling time: ~24 hours (Sigma-Aldrich).
Genetic integration: Method=ZFN; Gene=FPbase; DBBO8; mKate2 (Note=Bright red fluorescent protein derived from eqFP578).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
  • Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
Disease Childhood acute monocytic leukemia (NCIt: C9163)
Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0006 (THP-1)
Sex of cell Male
Age at sampling 1Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Sigma-Aldrich; CLL1223
Cell line databases/resources cancercelllines; CVCL_C3EW
Encyclopedic resources Wikidata; Q114313119
Entry history
Entry creation22-Sep-2022
Last entry update19-Dec-2024
Version number7