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Cellosaurus HNES1 GATA3:mKO2 (CVCL_C2VL)

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Cell line name HNES1 GATA3:mKO2
Synonyms HNES1_GATA3mKO2
Accession CVCL_C2VL
Resource Identification Initiative To cite this cell line use: HNES1 GATA3:mKO2 (RRID:CVCL_C2VL)
Comments Characteristics: Using CRISPR/Cas9 a IRES-mKO2-FRT-PGKp-Neo-PA construct was integrated following the stop codon of GATA3 (PubMed=33831366).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 134NN; mKusabira-Orange2 (Note=mKO2, orange fluorescent protein derived from F.concinna Kusabira-Orange (KO)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9R98 (CAMe001-A)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33831366; DOI=10.1016/j.stem.2021.02.025; PMCID=PMC8189439
Ge Guo, Giuliano Giuseppe Stirparo, Stanley E. Strawbridge, Daniel Spindlow, Jian Yang, James Clarke, Anish Dattani, Ayaka Yanagida, Meng Amy Li, Samuel Myers, Buse Nurten Ozel ...Show all 13 authors... , Jennifer Nichols, Austin Gerard Smith; Show fewer authors
Human naive epiblast cells possess unrestricted lineage potential.
Cell Stem Cell 28:1040-1056.e6(2021)

Cross-references
Encyclopedic resources Wikidata; Q114311634
Entry history
Entry creation22-Sep-2022
Last entry update14-Aug-2025
Version number6