Cellosaurus cell line STBCi322-A-1 (CVCL

Cellosaurus STBCi322-A-1 (CVCL_C1XW)

Cross-references
Cell line name	STBCi322-A-1
Synonyms	NKX6.1-GFP reporter line; NKX6.1-GFP clone 1a-10-3; AZi323-A-1
Accession	CVCL_C1XW
Resource Identification Initiative	To cite this cell line use: STBCi322-A-1 (RRID:CVCL_C1XW)
Comments	From: AstraZeneca; Cambridge; United Kingdom. Characteristics: Using CRISPR/Cas9 a T2A-GFP-LoxP-PGKp-Neo-LoxP construct was introduced at the C-terminus of NKX6-1. Subsequently the LoxP-PGKp-Neo-LoxP sequence was removed by Cre-mediated excision (PubMed=29734117). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Derived from site: In situ; Skin; UBERON=UBERON_0002097. Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_ZX55 (STBCi322-A)
Sex of cell	Female
Age at sampling	36Y
Category	Induced pluripotent stem cell
Publications	PubMed=29734117; DOI=10.1016/j.scr.2018.04.010 Shailesh Kumar Gupta, Agata Wesolowska-Andersen, Anna Kirstine Ringgaard, Himjyot Jaiswal, Lu-Yan Song, Benoit Hastoy, Camilla Ingvorsen, Amir Taheri-Ghahfarokhi, Bjorn Magnusson, Marcello Maresca, Rikke R. Jensen ...Show all 20 authors... , Nicola L. Beer, Johannes Josef Fels, Lars Groth Grunnet, Melissa K. Thomas, Anna L. Gloyn, Ryan Hicks, Mark I. McCarthy, Mattias Hansson, Christian Honore; Show fewer authors NKX6.1 induced pluripotent stem cell reporter lines for isolation and analysis of functionally relevant neuronal and pancreas populations. Stem Cell Res. 29:220-231(2018)
Cell line databases/resources	hPSCreg; STBCi322-A-1
Encyclopedic resources	Wikidata; Q114313049
Entry history
Entry creation	22-Sep-2022
Last entry update	14-Aug-2025
Version number	6