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Cellosaurus U-2OS-Cas9-4 (CVCL_C0ZD)

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Cell line name U-2OS-Cas9-4
Accession CVCL_C0ZD
Resource Identification Initiative To cite this cell line use: U-2OS-Cas9-4 (RRID:CVCL_C0ZD)
Comments Population: Caucasian.
Characteristics: Transfected with a Cas9-T2A-PuroR construct.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=35445373; DOI=10.1007/s11010-022-04434-z
Yong-Hua Zhou, Ying-Jie Li, Yu Zhang, Zi-Lin Yi, Ming-Gui Jiang;
Establishment and application of a human osteosarcoma U-2OS cell line that can stably express Cas9 protein.
Mol. Cell. Biochem. 477:2183-2191(2022)

Cross-references
Encyclopedic resources Wikidata; Q114313141
Entry history
Entry creation22-Sep-2022
Last entry update14-Aug-2025
Version number9