ID   U-2OS-Cas9-4
AC   CVCL_C0ZD
DR   Wikidata; Q114313141
RX   PubMed=35445373;
CC   Population: Caucasian.
CC   Characteristics: Transfected with a Cas9-T2A-PuroR construct.
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 22-09-22; Last updated: 14-08-25; Version: 9
//
RX   PubMed=35445373; DOI=10.1007/s11010-022-04434-z;
RA   Zhou, Yong-Hua
RA   Li, Ying-Jie
RA   Zhang, Yu
RA   Yi, Zi-Lin
RA   Jiang, Ming-Gui
RT   "Establishment and application of a human osteosarcoma U-2OS cell line
RT   that can stably express Cas9 protein.";
RL   Mol. Cell. Biochem. 477:2183-2191(2022).
//