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Cellosaurus BIONi010-C-NKX2.5-T2A-Nluc-73.59 (CVCL_C0VU)

[Text version]
Cell line name BIONi010-C-NKX2.5-T2A-Nluc-73.59
Synonyms #73.59
Accession CVCL_C0VU
Resource Identification Initiative To cite this cell line use: BIONi010-C-NKX2.5-T2A-Nluc-73.59 (RRID:CVCL_C0VU)
Comments Population: African.
Biotechnology: Part of the PluriLum assay which is used for predicting developmental toxicity which relies on the formation of embryoid bodies (EBs) and the differentiation of these into cardiomyocytes (PubMed=33660062).
Characteristics: Using CRISPR/Cas9 a T2A-Nluc construct was inserted at the C-terminus of both alleles of NKX2-5 (PubMed=33660062).
Transfected with: NanoLuc, a 19 kDa ATP-independent bright luminescent protein derived from Oplophorus gracilirostris.
Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1E68 (BIONi010-C)
Sex of cell Male
Age at sampling 18Y
Category Induced pluripotent stem cell

PubMed=33660062; DOI=10.1007/s00204-021-03018-y
Lauschke K., Treschow A.F., Rasmussen M.A., Davidsen N., Holst B., Emneus J., Taxvig C., Vinggaard A.M.
Creating a human-induced pluripotent stem cell-based NKX2.5 reporter gene assay for developmental toxicity testing.
Arch. Toxicol. 95:1659-1670(2021)

Encyclopedic resources Wikidata; Q114310753
Entry history
Entry creation22-Sep-2022
Last entry update29-Jun-2023
Version number4