Cellosaurus SKLRMe002-A (CVCL_C0JX)
| Cell line name | SKLRMe002-A |
|---|---|
| Synonyms | hEPS1-iCas9-B |
| Accession | CVCL_C0JX |
| Resource Identification Initiative | To cite this cell line use: SKLRMe002-A (RRID:CVCL_C0JX) |
| Comments | From: State Key Laboratory of Reproductive Medicine, Nanjing Medical University; Nanjing; China. Characteristics: Using CRISPR/Cas9 two different constructs were introduced in the two alleles of the AAVS1 safe harbor locus. In one allele a doxycycline inducible PuroR-Cas9 cassette was introduced while in the other alleles a constitutive reverse tetracycline trans-activator (M2rtTA) expression cassette Neo-M2rtTA was introduced (PubMed=35617843). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=35617843; DOI=10.1016/j.scr.2022.102816 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; SKLRMe002-A |
| Encyclopedic resources | Wikidata; Q112930409 |
| Entry history | |
| Entry creation | 23-Jun-2022 |
| Last entry update | 29-Jun-2023 |
| Version number | 4 |