ID   SKLRMe002-A
AC   CVCL_C0JX
SY   hEPS1-iCas9-B
DR   hPSCreg; SKLRMe002-A
DR   Wikidata; Q112930409
RX   PubMed=35617843;
CC   From: State Key Laboratory of Reproductive Medicine, Nanjing Medical University; Nanjing; China.
CC   Characteristics: Using CRISPR/Cas9 two different constructs were introduced in the two alleles of the AAVS1 safe harbor locus. In one allele a doxycycline inducible PuroR-Cas9 cassette was introduced while in the other alleles a constitutive reverse tetracycline trans-activator (M2rtTA) expression cassette Neo-M2rtTA was introduced (PubMed=35617843).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 23-06-22; Last updated: 29-06-23; Version: 4
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RX   PubMed=35617843; DOI=10.1016/j.scr.2022.102816;
RA   He Z.-Z., Feng K., Sun H., Yu T.-Y., Zhu D.-C., Yang Y.;
RT   "Generation of a human extended pluripotent stem cell line
RT   (SKLRMe002-A) carrying a doxycycline-inducible Cas9 expression
RT   cassette.";
RL   Stem Cell Res. 62:102816-102816(2022).
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