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Cellosaurus SCVIi038-A (CVCL_C0J0)

[Text version]
Cell line name SCVIi038-A
Synonyms AICS-0052-003-iKRABdCas9; ipsc WTC-my17 KRAB-mcherry clone 1
Accession CVCL_C0J0
Resource Identification Initiative To cite this cell line use: SCVIi038-A (RRID:CVCL_C0J0)
Comments From: Stanford Cardiovascular Institute; Palo Alto; USA.
Population: Japanese.
Characteristics: Using CRISPR/Cas9 a doxycycline-inducible dCas9-KRAB-mCherry cassette was introduced in both alleles of the AAVS1 safe harbor locus (PubMed=35325819).
Characteristics: Using CRISPR/Cas9 MYL7 has been endogenously tagged in one allele at the C-terminus with mEGFP.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UD15 (WTC-mEGFP-MYL7-cl3)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Publications

PubMed=35325819; DOI=10.1016/j.scr.2022.102754
Metzl-Raz E., Bharucha N., Arthur Ataam J., Gavidia A.A., Greenleaf W.J., Karakikes I.
Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9.
Stem Cell Res. 61:102754-102754(2022)

Cross-references
Cell line databases/resources hPSCreg; SCVIi038-A
Encyclopedic resources Wikidata; Q112930358
Entry history
Entry creation23-Jun-2022
Last entry update30-Jan-2024
Version number5