ID   SCVIi038-A
AC   CVCL_C0J0
SY   AICS-0052-003-iKRABdCas9; ipsc WTC-my17 KRAB-mcherry clone 1
DR   hPSCreg; SCVIi038-A
DR   Wikidata; Q112930358
RX   PubMed=35325819;
CC   From: Stanford Cardiovascular Institute; Palo Alto; USA.
CC   Population: Japanese.
CC   Characteristics: Using CRISPR/Cas9 a doxycycline-inducible dCas9-KRAB-mCherry cassette was introduced in both alleles of the AAVS1 safe harbor locus (PubMed=35325819).
CC   Characteristics: Using CRISPR/Cas9 MYL7 has been endogenously tagged in one allele at the C-terminus with mEGFP.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; QKFJN; mEGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
CC   Cell type: Fibroblast of skin; CL=CL_0002620.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_UD15 ! WTC-mEGFP-MYL7-cl3
SX   Male
AG   30Y
CA   Induced pluripotent stem cell
DT   Created: 23-06-22; Last updated: 10-04-25; Version: 7
//
RX   PubMed=35325819; DOI=10.1016/j.scr.2022.102754; PMCID=PMC9169598;
RA   Metzl-Raz E., Bharucha N., Arthur Ataam J., Gavidia A.A.,
RA   Greenleaf W.J., Karakikes I.;
RT   "Generation of a dual edited human induced pluripotent stem cell
RT   Myl7-GFP reporter line with inducible CRISPRi/dCas9.";
RL   Stem Cell Res. 61:102754-102754(2022).
//