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Cellosaurus MRIi003-A-6 (CVCL_C0IZ)

[Text version]
Cell line name MRIi003-A-6
Synonyms AAVS-CAG-VSFP homozygous; AAVS1-CAG-VSFP-homo
Accession CVCL_C0IZ
Resource Identification Initiative To cite this cell line use: MRIi003-A-6 (RRID:CVCL_C0IZ)
Comments From: Klinikum rechts der Isar, Technical University of Munich; Munich; Germany.
Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a CAG-promoter driven voltage-sensing fluorescent protein (VSFP) that consist of of a voltage-sensing transmembrane domain fused to a pair of fluorescent proteins: Clover and mRuby2 was integrated into both alleles of the AAVS1 safe harbor locus (PubMed=35421847).
Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Ser65Gly, p.Gln69Ala, p.Phe99Ser, p.Asn105Phe, p.Tyr145Phe, p.Met153Thr, p.Val163Ala, p.Ile171Val and p.Thr203His = Clover GFP).
Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby2).
Misspelling: MRli003-A-6; PubMed=35421847.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A8MN (MRIi003-A)
Sex of cell Male
Age at sampling Age unspecified
Category Induced pluripotent stem cell
Publications

PubMed=35421847; DOI=10.1016/j.scr.2022.102785
Zhang F.-F., Meier A.B., Sinnecker D., Engelhardt S., Lipp P., Laugwitz K.-L., Dorn T., Moretti A.
Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus.
Stem Cell Res. 61:102785-102785(2022)

Cross-references
Cell line databases/resources hPSCreg; MRIi003-A-6
Biological sample resources BioSamples; SAMEA10056290
Encyclopedic resources Wikidata; Q112930127
Entry history
Entry creation23-Jun-2022
Last entry update30-Jan-2024
Version number6