ID   MRIi003-A-6
AC   CVCL_C0IZ
SY   AAVS-CAG-VSFP homozygous; AAVS1-CAG-VSFP-homo
DR   BioSamples; SAMEA10056290
DR   hPSCreg; MRIi003-A-6
DR   Wikidata; Q112930127
RX   PubMed=35421847;
CC   From: Klinikum rechts der Isar, Technical University of Munich; Munich; Germany.
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 a CAG-promoter driven voltage-sensing fluorescent protein (VSFP) that consist of of a voltage-sensing transmembrane domain fused to a pair of fluorescent proteins: Clover and mRuby2 was integrated into both alleles of the AAVS1 safe harbor locus (PubMed=35421847).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Ser30Arg, p.Tyr39Asn, p.Ser65Gly, p.Gln69Ala, p.Phe99Ser, p.Asn105Phe, p.Tyr145Phe, p.Met153Thr, p.Val163Ala, p.Ile171Val and p.Thr203His = Clover GFP).
CC   Transfected with: UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby2).
CC   Misspelling: MRli003-A-6; PubMed=35421847.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A8MN ! MRIi003-A
SX   Male
AG   Age unspecified
CA   Induced pluripotent stem cell
DT   Created: 23-06-22; Last updated: 30-01-24; Version: 6
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RX   PubMed=35421847; DOI=10.1016/j.scr.2022.102785;
RA   Zhang F.-F., Meier A.B., Sinnecker D., Engelhardt S., Lipp P.,
RA   Laugwitz K.-L., Dorn T., Moretti A.;
RT   "Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6)
RT   voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of
RT   the AAVS1 locus.";
RL   Stem Cell Res. 61:102785-102785(2022).
//