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Cellosaurus SCVIi028-A-1 (CVCL_C0EX)

[Text version]
Cell line name SCVIi028-A-1
Synonyms SCVi-15S-PE2
Accession CVCL_C0EX
Resource Identification Initiative To cite this cell line use: SCVIi028-A-1 (RRID:CVCL_C0EX)
Comments From: Stanford Cardiovascular Institute; Palo Alto; USA.
Characteristics: Dox-inducible expression of the prime editor protein (PE2) which is a fusion between the S.pyogenes Cas9(H840A) and an engineered Moloney murine leukemia virus (MMLV) reverse transcriptase domain. PE2 enables gene editing at potentially any locus in the genome by transfecting a pegRNA specific for the desired target locus (PubMed=34875545).
Characteristics: Using CRISPR/Cas9 a T2A-Neo-CAG-rtTA-BFP-P2A-PE2-TRE3G construct has been inserted at the safe harbor locus (AAVS1) (PubMed=34875545).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.His840Ala).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Transfected with: UniProtKB; P42212; GFP (with p.Tyr66His and p.Tyr145Phe = BFP).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C0EW (SCVIi028-A)
Sex of cell Male
Age at sampling 21Y
Category Induced pluripotent stem cell
Publications

PubMed=34875545; DOI=10.1016/j.scr.2021.102610
Bharucha N., Ataam J.A., Gavidia A.A., Karakikes I.
Generation of AAVS1 integrated doxycycline-inducible CRISPR-Prime Editor human induced pluripotent stem cell line.
Stem Cell Res. 57:102610-102610(2021)

Cross-references
Cell line databases/resources hPSCreg; SCVIi028-A-1
Encyclopedic resources Wikidata; Q112930355
Entry history
Entry creation23-Jun-2022
Last entry update05-Oct-2023
Version number5