ID   SCVIi028-A-1
AC   CVCL_C0EX
SY   SCVi-15S-PE2
DR   hPSCreg; SCVIi028-A-1
DR   Wikidata; Q112930355
RX   PubMed=34875545;
CC   From: Stanford Cardiovascular Institute; Palo Alto; USA.
CC   Characteristics: Dox-inducible expression of the prime editor protein (PE2) which is a fusion between the S.pyogenes Cas9(H840A) and an engineered Moloney murine leukemia virus (MMLV) reverse transcriptase domain. PE2 enables gene editing at potentially any locus in the genome by transfecting a pegRNA specific for the desired target locus (PubMed=34875545).
CC   Characteristics: Using CRISPR/Cas9 a T2A-Neo-CAG-rtTA-BFP-P2A-PE2-TRE3G construct has been inserted at the AAVS1 safe harbor locus (PubMed=34875545).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; SDS4K; BFP (Note=Blue fluorescent protein derived from GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.His840Ala).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C0EW ! SCVIi028-A
SX   Male
AG   21Y
CA   Induced pluripotent stem cell
DT   Created: 23-06-22; Last updated: 10-04-25; Version: 8
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RX   PubMed=34875545; DOI=10.1016/j.scr.2021.102610; PMCID=PMC9126997;
RA   Bharucha N., Arthur Ataam J., Gavidia A.A., Karakikes I.;
RT   "Generation of AAVS1 integrated doxycycline-inducible CRISPR-Prime
RT   Editor human induced pluripotent stem cell line.";
RL   Stem Cell Res. 57:102610-102610(2021).
//