ID   SCVIi028-A-1
AC   CVCL_C0EX
SY   SCVi-15S-PE2
DR   hPSCreg; SCVIi028-A-1
DR   Wikidata; Q112930355
RX   PubMed=34875545;
CC   From: Stanford Cardiovascular Institute; Palo Alto; USA.
CC   Characteristics: Dox-inducible expression of the prime editor protein (PE2) which is a fusion between the S.pyogenes Cas9(H840A) and an engineered Moloney murine leukemia virus (MMLV) reverse transcriptase domain. PE2 enables gene editing at potentially any locus in the genome by transfecting a pegRNA specific for the desired target locus (PubMed=34875545).
CC   Characteristics: Using CRISPR/Cas9 a T2A-Neo-CAG-rtTA-BFP-P2A-PE2-TRE3G construct has been inserted at the safe harbor locus (AAVS1) (PubMed=34875545).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.His840Ala).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Tyr66His and p.Tyr145Phe = BFP).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C0EW ! SCVIi028-A
SX   Male
AG   21Y
CA   Induced pluripotent stem cell
DT   Created: 23-06-22; Last updated: 05-10-23; Version: 5
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RX   PubMed=34875545; DOI=10.1016/j.scr.2021.102610;
RA   Bharucha N., Ataam J.A., Gavidia A.A., Karakikes I.;
RT   "Generation of AAVS1 integrated doxycycline-inducible CRISPR-Prime
RT   Editor human induced pluripotent stem cell line.";
RL   Stem Cell Res. 57:102610-102610(2021).
//