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Cellosaurus U-2 OS-CRISPR-NUP96-SNAP clone #33 (CVCL_B7FL)

[Text version]
Cell line name U-2 OS-CRISPR-NUP96-SNAP clone #33
Accession CVCL_B7FL
Resource Identification Initiative To cite this cell line use: U-2 OS-CRISPR-NUP96-SNAP clone #33 (RRID:CVCL_B7FL)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9D10A NUP96 has been endogenously tagged in both alleles at the C-terminus with a SNAP-tag (PubMed=31562488).
Transfected with: HGNC; 7059; MGMT (with p.Gly180_Asn_207del and 19 mutations = SNAP-tag).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=31562488; DOI=10.1038/s41592-019-0574-9
Thevathasan J.V., Kahnwald M., Cieslinski K., Hoess P., Peneti S.K., Reitberger M., Heid D., Kasuba K.C., Hoerner S.J., Li Y.-M., Wu Y.-L., Mund M., Matti U., Pereira P.M., Henriques R., Nijmeijer B., Kueblbeck M., Sabinina V.J., Ellenberg J., Ries J.
Nuclear pores as versatile reference standards for quantitative superresolution microscopy.
Nat. Methods 16:1045-1053(2019)

Cross-references
Cell line collections (Providers) CLS; 300444
Encyclopedic resources Wikidata; Q112930492
Entry history
Entry creation23-Jun-2022
Last entry update05-Oct-2023
Version number5