ID   U-2 OS-CRISPR-NUP96-SNAP clone #33
AC   CVCL_B7FL
DR   CLS; 300444
DR   Wikidata; Q112930492
RX   PubMed=31562488;
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9D10A NUP96 has been endogenously tagged in both alleles at the C-terminus with a SNAP-tag (PubMed=31562488).
CC   Sequence variation: Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag).
CC   Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0042 ! U2OS
SX   Female
AG   15Y
CA   Cancer cell line
DT   Created: 23-06-22; Last updated: 19-12-24; Version: 7
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RX   PubMed=31562488; DOI=10.1038/s41592-019-0574-9; PMCID=PMC6768092;
RA   Thevathasan J.V., Kahnwald M., Cieslinski K., Hoess P., Peneti S.K.,
RA   Reitberger M., Heid D., Kasuba K.C., Hoerner S.J., Li Y.-M., Wu Y.-L.,
RA   Mund M., Matti U., Pereira P.M., Henriques R., Nijmeijer-Winter B.,
RA   Kueblbeck M., Sabinina V.J., Ellenberg J., Ries J.;
RT   "Nuclear pores as versatile reference standards for quantitative
RT   superresolution microscopy.";
RL   Nat. Methods 16:1045-1053(2019).
//