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Cellosaurus WA09 CRX+/tdTomato (CVCL_B5ZJ)

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Cell line name WA09 CRX+/tdTomato
Synonyms CRX+/tdTomato
Accession CVCL_B5ZJ
Resource Identification Initiative To cite this cell line use: WA09 CRX+/tdTomato (RRID:CVCL_B5ZJ)
Comments Characteristics: Using BAC homologous recombination a single allele of CRX was replaced with a tdTomato reporter gene (PubMed=29230913).
Knockout cell: Method=BAC homologous recombination; HGNC; HGNC:2383; CRX (Note=1 of 2 alleles).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9773 (WA09)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=29230913; DOI=10.1002/stem.2755; PMCID=PMC5823737
M. Joseph Phillips, Peng Jiang, Sara E. Howden, Patrick Barney, Jee Min, Nathaniel W. York, Li-Fang Chu, Elizabeth E. Capowski, Abigail Cash, Shivani Jain, Katherine Barlow ...Show all 16 authors... , Tasnia Tabassum, Ron Stewart, Bikash R. Pattnaik, James Alexander Thomson, David Matthew Gamm; Show fewer authors
A novel approach to single cell RNA-sequence analysis facilitates in silico gene reporting of human pluripotent stem cell-derived retinal cell types.
Stem Cells 36:313-324(2018)

Cross-references
Encyclopedic resources Wikidata; Q112041916
Entry history
Entry creation17-Mar-2022
Last entry update19-Dec-2024
Version number7