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Cellosaurus HVRDe008-A-2 (CVCL_B5S4)

[Text version]
Cell line name HVRDe008-A-2
Synonyms GAPDH-Cas9-Puro clone #28; GC9P clone #28
Accession CVCL_B5S4
Resource Identification Initiative To cite this cell line use: HVRDe008-A-2 (RRID:CVCL_B5S4)
Comments Characteristics: Using CRISPR/Cas9 a P2A-eSpCas9-IRES-PuroR-pA construct was introduced at the C-terminus of one allele of GAPDH (PubMed=34741877).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B207 (HUES 8)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell

PubMed=34741877; DOI=10.1016/j.scr.2021.102591
Sintov E., Gerace D., Melton D.A.
A human ESC line for efficient CRISPR editing of pluripotent stem cells.
Stem Cell Res. 57:102591-102591(2021)

Cell line databases/resources hPSCreg; HVRDe008-A-2
Biological sample resources BioSamples; SAMEA10271031
Encyclopedic resources Wikidata; Q111733467
Entry history
Entry creation17-Mar-2022
Last entry update29-Jun-2023
Version number4