ID   HVRDe008-A-2
AC   CVCL_B5S4
SY   GAPDH-Cas9-Puro clone #28; GC9P clone #28
DR   BioSamples; SAMEA10271031
DR   hPSCreg; HVRDe008-A-2
DR   Wikidata; Q111733467
RX   PubMed=34741877;
CC   Characteristics: Using CRISPR/Cas9 a P2A-eSpCas9-IRES-PuroR-pA construct was introduced at the C-terminus of one allele of GAPDH (PubMed=34741877).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_B207 ! HUES 8
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 17-03-22; Last updated: 29-06-23; Version: 4
//
RX   PubMed=34741877; DOI=10.1016/j.scr.2021.102591;
RA   Sintov E., Gerace D., Melton D.A.;
RT   "A human ESC line for efficient CRISPR editing of pluripotent stem
RT   cells.";
RL   Stem Cell Res. 57:102591-102591(2021).
//