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Cellosaurus HUES 15 (CVCL_B145)

[Text version]
Cell line name HUES 15
Synonyms HUES-15; HUES15; HuES15; HVRDe015-A
Accession CVCL_B145
Resource Identification Initiative To cite this cell line use: HUES 15 (RRID:CVCL_B145)
Comments From: Harvard University; Boston; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0028.
Registration: UK Stem Cell Bank (UKSCB); R-06-015, Steering comm. appl. SCSC04-25(15).
Doubling time: 48 hours (PubMed=14999088).
Donor information: Embryo is sibling to that giving rise to HUES 14 (Cellosaurus=CVCL_B144).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=19200798

Markers:
AmelogeninX
CSF1PO10,12
D3S135817,18
D5S81811,12
D7S82010
D8S117911,13
D13S3179
D16S5399,10
D18S5113,15
D21S1128,30
FGA20,21
Penta D10,12
Penta E16,18
TH017
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Publications

PubMed=14999088; DOI=10.1056/NEJMsr040330
Cowan C.A., Klimanskaya I.V., McMahon J., Atienza J., Witmyer J., Zucker J.P., Wang S.-P., Morton C.C., McMahon A.P., Powers D., Melton D.A.
Derivation of embryonic stem-cell lines from human blastocysts.
N. Engl. J. Med. 350:1353-1356(2004)

PubMed=17572666; DOI=10.1038/nbt1318
Adewumi O., Aflatoonian B., Ahrlund-Richter L., Amit M., Andrews P.W., Beighton G., Bello P.A., Benvenisty N., Berry L.S., Bevan S., Blum B., Brooking J., Chen K.G., Choo A.B.-H., Churchill G.A., Corbel M., Damjanov I., Draper J.S., Dvorak P., Emanuelsson K., Fleck R.A., Ford A., Gertow K., Gertsenstein M., Gokhale P.J., Hamilton R.S., Hampl A., Healy L.E., Hovatta O., Hyllner J., Imreh M.P., Itskovitz-Eldor J., Jackson J., Johnson J.L., Jones M., Kee K., King B.L., Knowles B.B., Lako M., Lebrin F., Mallon B.S., Manning D., Mayshar Y., McKay R.D.G., Michalska A.E., Mikkola M., Mileikovsky M., Minger S.L., Moore H.D., Mummery C.L., Nagy A., Nakatsuji N., O'Brien C.M., Oh S.K.-W., Olsson C., Otonkoski T., Park K.-Y., Passier R., Patel H., Patel M.J., Pedersen R., Pera M.F., Piekarczyk M.S., Reijo Pera R.A., Reubinoff B.E., Robins A.J., Rossant J., Rugg-Gunn P., Schulz T.C., Semb H., Sherrer E.S., Siemen H., Stacey G.N., Stojkovic M., Suemori H., Szatkiewicz J., Turetsky T.T., Tuuri T., van den Brink S., Vintersten K., Vuoristo S., Ward-van Oostwaard D., Weaver T.A., Young L.A., Zhang W.-D.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=18772870; DOI=10.1038/nprot.2008.140
Braam S.R., Denning C., Matsa E., Young L.E., Passier R., Mummery C.L.
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification.
Nat. Protoc. 3:1435-1443(2008)

PubMed=19200798; DOI=10.1016/j.stem.2008.12.001
Chen A.E., Egli D., Niakan K.K., Deng J., Akutsu H., Yamaki M., Cowan C.A., Fitz-Gerald C., Zhang K., Melton D.A., Eggan K.C.
Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Cell Stem Cell 4:103-106(2009)

Cross-references
Cell line databases/resources hPSCreg; HVRDe015-A
ISCR; 116
NIHhESC; NIHhESC-10-0028
SKIP; SKIP001498
SKIP; SKIP001919
Encyclopedic resources Wikidata; Q54896744
Entry history
Entry creation06-Jun-2012
Last entry update29-Jun-2023
Version number20