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Cellosaurus HUES 1 (CVCL_B139)

[Text version]
Cell line name HUES 1
Synonyms HUES-1; HUES1; HuES1; Hues1; HuES01; HVRDe001-A
Accession CVCL_B139
Resource Identification Initiative To cite this cell line use: HUES 1 (RRID:CVCL_B139)
Comments From: Harvard University; Boston; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0014.
Registration: Swiss research registry; BAG-hES-IMP-0035.
Registration: UK Stem Cell Bank (UKSCB); R-05-033, Steering comm. appl. SCSC04-25(1).
Doubling time: 24 hours (PubMed=14999088).
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=19200798

Markers:
AmelogeninX
CSF1PO12
D3S135815,18
D5S81811,12
D7S82010
D8S117910,12
D13S31711,12
D16S5398,12
D18S5113
D21S1128,30
FGA21,23
Penta D9,12
Penta E7,10
TH017,9
TPOX8,9
vWA17,18

Run an STR similarity search on this cell line
Publications

PubMed=14999088; DOI=10.1056/NEJMsr040330
Chad A. Cowan, Irina V. Klimanskaya, Jill McMahon, Jocelyn Atienza, Jeannine Witmyer, Jacob P. Zucker, Shun-Ping Wang, Cynthia Casson Morton, Andrew P. McMahon, Doug Powers, Douglas A. Melton;
Derivation of embryonic stem-cell lines from human blastocysts.
N. Engl. J. Med. 350:1353-1356(2004)

PubMed=17572666; DOI=10.1038/nbt1318
Oluseun Adewumi, Behrouz Aflatoonian, Lars Ahrlund-Richter, Michal Amit, Peter W. Andrews, Gemma Beighton, Paul A. Bello, Nissim Benvenisty, Lorraine S. Berry, Simon Bevan, Barak Blum ...Show all 85 authors... , Justin Brooking, Kevin G. Chen, Andre B.-H. Choo, Gary A. Churchill, Marie Corbel, Ivan Damjanov, Jonathan S. Draper, Petr Dvorak, Katarina Emanuelsson, Roland A. Fleck, Angela Ford, Karin Gertow, Marina Gertsenstein, Paul J. Gokhale, Rebecca S. Hamilton, Ales Hampl, Lyn E. Healy, Outi Hovatta, Johan Hyllner, Marta P. Imreh, Joseph Itskovitz-Eldor, Jamie Jackson, Jacqueline L. Johnson, Mark Jones, Kehkooi Kee, Benjamin L. King, Barbara B. Knowles, Majlinda Lako, Franck Lebrin, Barbara S. Mallon, Daisy Manning, Yoav Mayshar, Ronald Davies Graham McKay, Anna E. Michalska, Milla Mikkola, Maria Mileikovsky, Stephen Lewis Minger, Harry D. Moore, Christine L. Mummery, Andras Nagy, Norio Nakatsuji, Carmel M. O'Brien, Steve Kah-Weng Oh, Cia Olsson, Timo Otonkoski, Kye-Yoon Park, Robert Passier, Hema Patel, Minal J. Patel, Roger Arnold Pedersen, Martin F. Pera, Marian S. Piekarczyk, Renee A. Reijo Pera, Benjamin E. Reubinoff, Allan J. Robins, Janet Rossant, Peter Rugg-Gunn, Thomas C. Schulz, Henrik Semb, Eric S. Sherrer, Henrike Siemen, Glyn N. Stacey, Miodrag Stojkovic, Hirofumi Suemori, Jin Szatkiewicz, Tikva Tako Turetsky, Timo Tuuri, Stieneke van den Brink, Kristina Vintersten, Sanna Vuoristo, Dorien Ward-van Oostwaard, Thomas A. Weaver, Lesley A. Young, Wei-Dong Zhang; Show fewer authors
International Stem Cell Initiative
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat. Biotechnol. 25:803-816(2007)

PubMed=18772870; DOI=10.1038/nprot.2008.140
Stefan R. Braam, Chris Denning, Elena Matsa, Lorraine E. Young, Robert Passier, Christine L. Mummery;
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification.
Nat. Protoc. 3:1435-1443(2008)

PubMed=19200798; DOI=10.1016/j.stem.2008.12.001; PMCID=PMC3335201
Alice E. Chen, Dietrich Meinrad Egli, Kathy K. Niakan, Jie Deng, Hidenori Akutsu, Mariko Yamaki, Chad A. Cowan, Claire Fitz-Gerald, Kun Zhang, Douglas A. Melton, Kevin Carl Eggan;
Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Cell Stem Cell 4:103-106(2009)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032; PMCID=PMC3063454
Christoph Bock, Evangelos Kiskinis, Griet Verstappen, Hong-Cang Gu, Gabriella L. Boulting, Zachary D. Smith, Michael Johannes Ziller, Gist Fralley Croft, Mackenzie Weygandt Amoroso, Derek H. Oakley, Andreas Gnirke ...Show all 13 authors... , Kevin Carl Eggan, Alexander Meissner; Show fewer authors
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=21714037; DOI=10.1002/stem.681
Jean-Marie Ramirez, Sabine Gerbal-Chaloin, Ollivier Milhavet, Bai Qiang, Fabienne Becker, Said Assou, Jean-Marc Lemaitre, Samir Hamamah, John De Vos;
Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
Stem Cells 29:1469-1474(2011)

Cross-references
Cell line databases/resources hPSCreg; HVRDe001-A
NIHhESC; NIHhESC-10-0014
SKIP; SKIP001019
SKIP; SKIP001905
Biological sample resources ENCODE; ENCBS771TSF
ENCODE; ENCBS790PAZ
ENCODE; ENCBS836MPJ
Encyclopedic resources Wikidata; Q54896736
Experimental variables resources EFO; EFO_0005355
Gene expression databases GEO; GSM637758
GEO; GSM1422443
GEO; GSM1422444
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number27