Cellosaurus HES-3 MESP1mCherry/w NKX2-5eGFP/w (CVCL_A8JT)
| Cell line name | HES-3 MESP1mCherry/w NKX2-5eGFP/w |
|---|---|
| Accession | CVCL_A8JT |
| Resource Identification Initiative | To cite this cell line use: HES-3 MESP1mCherry/w NKX2-5eGFP/w (RRID:CVCL_A8JT) |
| Comments | Characteristics: Useful for dissecting the molecular and cellular mechanisms related to the earliest steps in cardiac development in live cells (PubMed=25187301). Characteristics: Using homologous recombination an EGFP reporter gene was introduced at the first exon of one allele of the NKX2.5 locus. Characteristics: Using homologous recombination a mCherry reporter gene was introduced at the first exon of one allele of the MESP1 locus. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_A8JS (HES-3 NKX2.5eGFP/w) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=25187301; DOI=10.1002/stem.1842 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q108820461 |
| Entry history | |
| Entry creation | 23-Sep-2021 |
| Last entry update | 05-Oct-2023 |
| Version number | 5 |