Cellosaurus logo
expasy logo

Cellosaurus HES-3 NKX2.5eGFP/w (CVCL_A8JS)

[Text version]
Cell line name HES-3 NKX2.5eGFP/w
Synonyms HES-3 NKX2.5(eGFP/w); H3 NKX2-5GFP
Accession CVCL_A8JS
Resource Identification Initiative To cite this cell line use: HES-3 NKX2.5eGFP/w (RRID:CVCL_A8JS)
Comments Characteristics: Using homologous recombination an EGFP reporter gene was introduced at the first exon of one allele of the NKX2.5 locus (PubMed=22020065).
Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_7158 (HES-3)
Children:
CVCL_A8JT (HES-3 MESP1mCherry/w NKX2-5eGFP/w)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=22020065; DOI=10.1038/nmeth.1740
David A. Elliott, Stefan R. Braam, Katerina Koutsis, Elizabeth S. Ng, Robert Jenny, Ebba Louise Lagerqvist, Christine Biben, Tanya Hatzistavrou, Claire E. Hirst, Qing C. Yu, Rhys J.P. Skelton ...Show all 26 authors... , Dorien Ward-van Oostwaard, Sue Mei Lim, Ouda Khammy, Xue-Ling Li, Susan M. Hawes, Richard Paul Davis, Adam L. Goulburn, Robert Passier, Owen W.J. Prall, John M. Haynes, Colin William Pouton, David M. Kaye, Christine L. Mummery, Andrew George Elefanty, Edouard G. Stanley; Show fewer authors
NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
Nat. Methods 8:1037-1040(2011)

Cross-references
Encyclopedic resources Wikidata; Q108820462
Entry history
Entry creation23-Sep-2021
Last entry update14-Aug-2025
Version number6