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Cellosaurus HES-3 NKX2.5eGFP/w (CVCL_A8JS)

[Text version]
Cell line name HES-3 NKX2.5eGFP/w
Synonyms H3 NKX2-5GFP
Accession CVCL_A8JS
Resource Identification Initiative To cite this cell line use: HES-3 NKX2.5eGFP/w (RRID:CVCL_A8JS)
Comments Characteristics: Using homologous recombination an EGFP reporter gene was introduced at the first exon of one allele of the NKX2.5 locus.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_7158 (HES-3)
CVCL_A8JT (HES-3 MESP1mCherry/w NKX2-5eGFP/w)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell

PubMed=22020065; DOI=10.1038/nmeth.1740
Elliott D.A., Braam S.R., Koutsis K., Ng E.S., Jenny R., Lagerqvist E.L., Biben C., Hatzistavrou T., Hirst C.E., Yu Q.C., Skelton R.J.P., Ward-van Oostwaard D., Lim S.M., Khammy O., Li X.-L., Hawes S.M., Davis R.P., Goulburn A.L., Passier R., Prall O.W.J., Haynes J.M., Pouton C.W., Kaye D.M., Mummery C.L., Elefanty A.G., Stanley E.G.
NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
Nat. Methods 8:1037-1040(2011)

Encyclopedic resources Wikidata; Q108820462
Entry history
Entry creation23-Sep-2021
Last entry update29-Jun-2023
Version number4