Cellosaurus cell line HEK-Blue-Lucia Null (NF/IL8) (CVCL

Cellosaurus HEK-Blue-Lucia Null (NF/IL8) (CVCL_A8CQ)

[Text version]

Cell line name

HEK-Blue-Lucia Null (NF/IL8)

Synonyms

HEK-Blue-Lucia Null; HEK-Dual Null; HEK-Dual Null (NF/IL8); HEK-NF-KappaB-SEAP/KI-[IL-8]Lucia

Accession

CVCL_A8CQ

Resource Identification Initiative

To cite this cell line use: HEK-Blue-Lucia Null (NF/IL8) (RRID:CVCL_A8CQ)

Comments

Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites. The Lucia luciferase reporter gene expression depends on the activation of the endogenous CXCL8/IL8 promoter as the CXCL8 gene has been replaced by Lucia (InvivoGen=hkd-nullni).
Knockout cell: Method=Homologous recombination; HGNC; HGNC:6025; CXCL8.
Knockout cell: Method=Not specified; HGNC; HGNC:11849; TLR3.
Knockout cell: Method=Not specified; HGNC; HGNC:11851; TLR5.
Knockout cell: Method=Not specified; HGNC; HGNC:11916; TNFRSF1A.
Genetic integration: Method=Homologous recombination; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0045 (HEK293)
Children:

CVCL_A8CR (HEK-Blue-Lucia hTLR2 (NF/IL8))	CVCL_A8CS (HEK-Blue-Lucia hTLR3 (NF/IL8))	CVCL_A8CT (HEK-Blue-Lucia hTLR5 (NF/IL8))
CVCL_A8CU (HEK-Blue-Lucia hTLR9 (NF/IL8))	CVCL_A8CV (HEK-Blue-Lucia mTLR4 (NF/IL8))	CVCL_A8CW (HEK-Blue-Lucia mTLR7 (NF/IL8))

Sex of cell

Female

Age at sampling

Fetus