Cellosaurus HEK-Dual Null (CVCL_A8CQ)
| Cell line name | HEK-Dual Null | ||||||
|---|---|---|---|---|---|---|---|
| Synonyms | HEK-NF-KappaB-SEAP/KI-[IL-8]Lucia | ||||||
| Accession | CVCL_A8CQ | ||||||
| Resource Identification Initiative | To cite this cell line use: HEK-Dual Null (RRID:CVCL_A8CQ) | ||||||
| Comments | Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites. The Lucia luciferase reporter gene expression depends on the activation of the endogenous CXCL8/IL8 promoter as the CXCL8 gene has been replaced by Lucia. Knockout cell: Method=Not specified; HGNC; 6025; CXCL8. Knockout cell: Method=Not specified; HGNC; 11849; TLR3. Knockout cell: Method=Not specified; HGNC; 11851; TLR5. Knockout cell: Method=Not specified; HGNC; 11916; TNFRSF1A. Knockout cell: Method=Not specified; HGNC; 11917; TNFRSF1B. Transfected with: HGNC; 439; ALPP. Transfected with: Lucia luciferase, a synthetic gene designed on natural secreted luciferase genes from marine copepods. Transfected with: UniProtKB; P33967; B.cereus Bsr. Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. | ||||||
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) | ||||||
| Hierarchy | Parent: CVCL_0045 (HEK293) Children:
| ||||||
| Sex of cell | Female | ||||||
| Age at sampling | Fetus | ||||||
| Category | Transformed cell line | ||||||
| Web pages | https://www.invivogen.com/hek-dual-null | ||||||
| Cross-references | |||||||
| Encyclopedic resources | Wikidata; Q107115289 | ||||||
| Entry history | |||||||
| Entry creation | 20-May-2021 | ||||||
| Last entry update | 05-Oct-2023 | ||||||
| Version number | 7 | ||||||