ID   HEK-Blue-Lucia Null (NF/IL8)
AC   CVCL_A8CQ
SY   HEK-Blue-Lucia Null; HEK-Dual Null; HEK-Dual Null (NF/IL8); HEK-NF-KappaB-SEAP/KI-[IL-8]Lucia
DR   InvivoGen; hkd-nullni
DR   Wikidata; Q107115289
CC   Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites. The Lucia luciferase reporter gene expression depends on the activation of the endogenous CXCL8/IL8 promoter as the CXCL8 gene has been replaced by Lucia (InvivoGen=hkd-nullni).
CC   Knockout cell: Method=Homologous recombination; HGNC; HGNC:6025; CXCL8.
CC   Knockout cell: Method=Not specified; HGNC; HGNC:11849; TLR3.
CC   Knockout cell: Method=Not specified; HGNC; HGNC:11851; TLR5.
CC   Knockout cell: Method=Not specified; HGNC; HGNC:11916; TNFRSF1A.
CC   Genetic integration: Method=Homologous recombination; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 10
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