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Cellosaurus 293-Dual Null (CVCL_A8BK)

[Text version]
Cell line name 293-Dual Null
Synonyms 293-ISG-SEAP/KI-[IFN-beta]Lucia
Accession CVCL_A8BK
Resource Identification Initiative To cite this cell line use: 293-Dual Null (RRID:CVCL_A8BK)
Comments Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). The Lucia luciferase reporter gene expression depends on the activation of the endogenous IFNB1 promoter as the IFNB1 gene has been replaced by Lucia.
Knockout cell: Method=Not specified; HGNC; 5434; IFNB1.
Transfected with: HGNC; 439; ALPP.
Transfected with: Lucia luciferase, a synthetic gene designed on natural secreted luciferase genes from marine copepods.
Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Children:
CVCL_A8BL (293-Dual hSTING-A162)CVCL_A8BM (293-Dual hSTING-H232)CVCL_A8BN (293-Dual hSTING-R232)
CVCL_A8BP (293-Dual mSTING)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages https://www.invivogen.com/293-dual-null
Cross-references
Encyclopedic resources Wikidata; Q107113194
Entry history
Entry creation20-May-2021
Last entry update05-Oct-2023
Version number7