ID   293-Dual Null
AC   CVCL_A8BK
SY   293-ISG-SEAP/KI-[IFN-beta]Lucia
DR   Wikidata; Q107113194
WW   https://www.invivogen.com/293-dual-null
CC   Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). The Lucia luciferase reporter gene expression depends on the activation of the endogenous IFNB1 promoter as the IFNB1 gene has been replaced by Lucia.
CC   Knockout cell: Method=Not specified; HGNC; 5434; IFNB1.
CC   Transfected with: HGNC; 439; ALPP.
CC   Transfected with: Lucia luciferase, a synthetic gene designed on natural secreted luciferase genes from marine copepods.
CC   Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0045 ! HEK293
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 20-05-21; Last updated: 05-10-23; Version: 7
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