Cellosaurus cell line THP1-HMGB1-Lucia (CVCL

Cellosaurus THP1-HMGB1-Lucia (CVCL_A8BE)

Cross-references
Cell line name	THP1-HMGB1-Lucia
Accession	CVCL_A8BE
Resource Identification Initiative	To cite this cell line use: THP1-HMGB1-Lucia (RRID:CVCL_A8BE)
Comments	Population: Japanese. Characteristics: Designed to monitor ferroptosis, a form of non-apoptotic cell death. Stably express an HMGB1::Lucia luciferase fusion protein in the cytoplasm, following ferroptosis activation, the cell membrane ruptures and HMGB1::Lucia is released in the extracellular milieu (InvivoGen=thp1-gb1lc). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:4983; HMGB1. Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection; Gene=Synthetic construct Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations	Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line). Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line). Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
Disease	Childhood acute monocytic leukemia (NCIt: C9163) Acute monoblastic/monocytic leukemia (ORDO: Orphanet_514)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0006 (THP-1)
Sex of cell	Male
Age at sampling	1Y
Category	Cancer cell line
Cell line collections (Providers)	InvivoGen; thp-gb1lc
Cell line databases/resources	cancercelllines; CVCL_A8BE
Encyclopedic resources	Wikidata; Q107117083
Entry history
Entry creation	20-May-2021
Last entry update	14-Aug-2025
Version number	10