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Cellosaurus A549-Dual KO-MDA5 hACE2-TMPRSS2 (CVCL_A7ZR)

[Text version]
Cell line name A549-Dual KO-MDA5 hACE2-TMPRSS2
Accession CVCL_A7ZR
Resource Identification Initiative To cite this cell line use: A549-Dual KO-MDA5 hACE2-TMPRSS2 (RRID:CVCL_A7ZR)
Comments Group: SARS-CoV-2 research cell line.
Population: Caucasian.
Characteristics: Ideal for studying SARS-CoV-2 viral entry into host cells, the viral RNA sensing pathway, as well as screening small molecule inhibitors and neutralizing antibodies. These cells are permissive to infection by SARS-CoV-2 Spike-pseudotyped viral particles (InvivoGen).
Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE).
Knockout cell: Method=Not specified; HGNC; 18873; IFIH1.
Transfected with: HGNC; 13557; ACE2.
Transfected with: HGNC; 11876; TMPRSS2.
Transfected with: HGNC; 439; ALPP.
Transfected with: Lucia luciferase, a synthetic gene designed on natural secreted luciferase genes from marine copepods.
Transfected with: UniProtKB; P33967; B.cereus Bsr.
Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A7ZP (A549-Dual KO-MDA5)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Web pages https://www.invivogen.com/a549dual-hace2tmprss2-cells
Cell line databases/resources cancercelllines; CVCL_A7ZR
Encyclopedic resources Wikidata; Q107113330
Entry history
Entry creation20-May-2021
Last entry update30-Jan-2024
Version number8