Cellosaurus cell line A549-Dual KO-MDA5 (CVCL

Cellosaurus A549-Dual KO-MDA5 (CVCL_A7ZP)

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Cell line name

A549-Dual KO-MDA5

Accession

CVCL_A7ZP

Resource Identification Initiative

To cite this cell line use: A549-Dual KO-MDA5 (RRID:CVCL_A7ZP)

Comments

Population: Caucasian.
Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE) (InvivoGen=a549d-komda5).
Knockout cell: Method=Not specified; HGNC; HGNC:18873; IFIH1.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection; Gene=Synthetic construct Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.

Sequence variations

Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).

Disease

Lung adenocarcinoma (NCIt: C3512)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_5I73 (A549-Dual)
Children:

CVCL_A7ZR (A549-Dual KO-MDA5 hACE2-TMPRSS2)

Sex of cell

Male

Age at sampling

58Y