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Cellosaurus BHK21-MERS-Rep (CVCL_A7MT)

[Text version]
Cell line name BHK21-MERS-Rep
Accession CVCL_A7MT
Resource Identification Initiative To cite this cell line use: BHK21-MERS-Rep (RRID:CVCL_A7MT)
Comments Characteristics: Transfected with a pBAC-CMV-MERS-CoV replicon construct. The MERS-CoV replicon contains the genes encoding the MERS-CoV polyproteins 1a and 1b and the N protein, neo, and the renilla-type luciferase (Rluc) gene (PubMed=33616893).
Transfected with: UniProtKB; K9N7C7; Middle East respiratory syndrome-related coronavirus replicase polyprotein 1ab.
Transfected with: UniProtKB; K9N4V7; Middle East respiratory syndrome-related coronavirus nucleoprotein (N).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transfected with: UniProtKB; P27652; Renilla-type luciferase.
Derived from sampling site: Kidney.
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy Parent: CVCL_1915 (BHK-21 clone 13)
Sex of cell Male
Age at sampling 1D
Category Spontaneously immortalized cell line

PubMed=33616893; DOI=10.1007/s12250-020-00341-z
Chen J., Hu B.-J., Zhao K., Luo Y., Lin H.-F., Shi Z.-L.
Development of a MERS-CoV replicon cell line for antiviral screening.
Virol. Sin. 36:730-735(2021)

Encyclopedic resources Wikidata; Q107113463
Entry history
Entry creation20-May-2021
Last entry update23-Sep-2021
Version number2