ID   BHK21-MERS-Rep
AC   CVCL_A7MT
DR   Wikidata; Q107113463
RX   PubMed=33616893;
CC   Characteristics: Transfected with a pBAC-CMV-MERS-CoV replicon construct. The MERS-CoV replicon contains the genes encoding the MERS-CoV polyproteins 1a and 1b and the N protein, neo, and the Renilla reniformis renilla-type luciferase (Rluc) gene (PubMed=33616893).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; K9N4V7; Middle East respiratory syndrome-related coronavirus (MERS-CoV) nucleoprotein (N).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; K9N7C7; Middle East respiratory syndrome-related coronavirus (MERS-CoV) replicase polyprotein 1ab.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla reniformis renilla-type luciferase.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=10036; ! Mesocricetus auratus (Golden hamster)
HI   CVCL_1915 ! BHK-21 clone 13
SX   Male
AG   1D
CA   Spontaneously immortalized cell line
DT   Created: 20-05-21; Last updated: 14-08-25; Version: 8
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RX   PubMed=33616893; DOI=10.1007/s12250-020-00341-z; PMCID=PMC7898024;
RA   Chen, Jing
RA   Hu, Bing-Jie
RA   Zhao, Kai
RA   Luo, Yun
RA   Lin, Hao-Feng
RA   Shi, Zheng-Li
RT   "Development of a MERS-CoV replicon cell line for antiviral
RT   screening.";
RL   Virol. Sin. 36:730-735(2021).
//